Nalaganje...
A new method for determining the absolute molarity of solutions of trypsin and chymotrypsin by using p-nitrophenyl N(2)-acetyl-N(1)-benzylcarbazate
1. p-Nitrophenyl N(2)-acetyl-N(1)-benzylcarbazate (NPABC) was synthesized and shown to acylate α-chymotrypsin stoicheiometrically; reaction at 25° occurs almost instantaneously at pH7·04 and within 2min. at pH5·04 and there is no observable turnover during 10min. 2. The absolute molarity of solution...
Shranjeno v:
| Main Authors: | , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
1968
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1198616/ https://ncbi.nlm.nih.gov/pubmed/5642613 |
| Oznake: |
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