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A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells
The advent of RNA interference has led to the ability to interfere with gene expression and greatly expanded our ability to perform genetic screens in mammalian cells. The expression of short hairpin RNA (shRNA) from polymerase III promoters can be encoded in transgenes and used to produce small int...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2005
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1196357/ https://ncbi.nlm.nih.gov/pubmed/16141338 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0506306102 |
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