Fluorescence Polarization Method To Characterize Macrolide-Ribosome Interactions

A fluorescence polarization assay is described that measures the binding of fluorescently labeled erythromycin to 70S ribosomes from Escherichia coli and the displacement of the erythromycin from these ribosomes. The assay has been validated with several macrolide derivatives and other known antibio...

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Autores principales: Yan, Kang, Hunt, Eric, Berge, John, May, Earl, Copeland, Robert A., Gontarek, Richard R.
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology 2005
Materias:
Analytical Procedures
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC1196252/
https://ncbi.nlm.nih.gov/pubmed/16048949
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.49.8.3367-3372.2005
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