Immobilization of Escherichia coli RNA Polymerase and Location of Binding Sites by Use of Chromatin Immunoprecipitation and Microarrays

The genome-wide location of RNA polymerase binding sites was determined in Escherichia coli using chromatin immunoprecipitation and microarrays (chIP-chip). Cross-linked chromatin was isolated in triplicate from rifampin-treated cells, and DNA bound to RNA polymerase was precipitated with an antibod...

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Detalhes bibliográficos
Main Authors: Herring, Christopher D., Raffaelle, Marni, Allen, Timothy E., Kanin, Elenita I., Landick, Robert, Ansari, Aseem Z., Palsson, Bernhard Ø.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2005
Assuntos:
Genomics and Proteomics
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1196165/
https://ncbi.nlm.nih.gov/pubmed/16109958
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.187.17.6166-6174.2005
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