Gene targeting using a promoterless gene trap vector (“targeted trapping”) is an efficient method to mutate a large fraction of genes

A powerful tool for postgenomic analysis of mammalian gene function is gene targeting in mouse ES cells. We report that homologous recombination using a promoterless gene trap vector (“targeting trapping”) yields targeting frequencies averaging above 50%, a significant increase compared with current...

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Bibliografiske detaljer
Main Authors: Friedel, Roland H., Plump, Andrew, Lu, Xiaowei, Spilker, Kerri, Jolicoeur, Christine, Wong, Karen, Venkatesh, Tadmiri R., Yaron, Avraham, Hynes, Mary, Chen, Bin, Okada, Ami, McConnell, Susan K., Rayburn, Helen, Tessier-Lavigne, Marc
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 2005
Fag:
Biological Sciences
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1193537/
https://ncbi.nlm.nih.gov/pubmed/16129827
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0505474102
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