Crystallization of a mammalian membrane protein overexpressed in Saccharomyces cerevisiae

The Ca(2+)-ATPase SERCA1a (sarcoplasmic–endoplasmic reticulum Ca(2+)-ATPase isoform 1a) from rabbit has been overexpressed in Saccharomyces cerevisiae. This membrane protein was purified by avidin agarose affinity chromatography based on natural biotinylation in the expression host, followed by HPLC...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Jidenko, Marie, Nielsen, Rikke C., Sørensen, Thomas Lykke-Møller, Møller, Jesper V., le Maire, Marc, Nissen, Poul, Jaxel, Christine
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: National Academy of Sciences 2005
Pynciau:
Biological Sciences
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC1187984/
https://ncbi.nlm.nih.gov/pubmed/16087876
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0503986102
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