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Crystallization of a mammalian membrane protein overexpressed in Saccharomyces cerevisiae
The Ca(2+)-ATPase SERCA1a (sarcoplasmic–endoplasmic reticulum Ca(2+)-ATPase isoform 1a) from rabbit has been overexpressed in Saccharomyces cerevisiae. This membrane protein was purified by avidin agarose affinity chromatography based on natural biotinylation in the expression host, followed by HPLC...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2005
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1187984/ https://ncbi.nlm.nih.gov/pubmed/16087876 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0503986102 |
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