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Headloop suppression PCR and its application to selective amplification of methylated DNA sequences
Selective amplification in PCR is principally determined by the sequence of the primers and the temperature of the annealing step. We have developed a new PCR technique for distinguishing related sequences in which additional selectivity is dependent on sequences within the amplicon. A 5′ extension...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2005
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1184225/ https://ncbi.nlm.nih.gov/pubmed/16091627 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gni120 |
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