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Multiplex PCR: use of heat-stable Thermus thermophilus RecA protein to minimize non-specific PCR products

In this paper we report that the inclusion of heat-resistant RecA protein from a thermophilic bacteria, Thermus thermophilus, and its cofactor (ATP) in PCR effectively eliminates non-specific PCR products. The effect of RecA protein, which catalyzes pairing between homologous DNA molecules with grea...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Shigemori, Yasushi, Mikawa, Tsutomu, Shibata, Takehiko, Oishi, Michio
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Oxford University Press 2005
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC1183492/
https://ncbi.nlm.nih.gov/pubmed/16087733
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gni111
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