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General ligands in affinity chromatography. Cofactor–substrate elution of enzymes bound to the immobilized nucleotides adenosine 5′-monophosphate and nicotinamide–adenine dinucleotide

1. Two different gels have been prepared suitable for the separation of a number of enzymes, in particular NAD(+)-dependent dehydrogenases, by affinity chromatography. For both the matrix used was Sepharose 4B. For preparation (a), NAD(+)–Sepharose, 6-aminohexanoic acid has been coupled to the gel b...

Täydet tiedot

Tallennettuna:
Bibliografiset tiedot
Päätekijät: Mosbach, K., Guilford, H., Ohlsson, R., Scott, M.
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: 1972
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC1178759/
https://ncbi.nlm.nih.gov/pubmed/4346743
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