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Affinity chromatography of nicotinamide–adenine dinucleotide-linked dehydrogenases on immobilized derivatives of the dinucleotide

1. Three established methods for immobilization of ligands through primary amino groups promoted little or no attachment of NAD(+) through the 6-amino group of the adenine residue. Two of these methods (coupling to CNBr-activated agarose and to carbodi-imide-activated carboxylated agarose derivative...

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Detalhes bibliográficos
Main Authors: Barry, Standish, O'Carra, Pádraig
Formato: Artigo
Idioma:Inglês
Publicado em: 1973
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1165874/
https://ncbi.nlm.nih.gov/pubmed/4360246
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