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Affinity chromatography of nicotinamide nucleotide-dependent dehydrogenases on immobilized nucleotide derivatives

A series of chemically-defined adenosine phosphate ligands attached to Sepharose 4B were used as active-site probes in studying the interaction of enzymes with their coenzymes and substrates and to test the suitability of these matrices for `general ligand' affinity chromatography. Nicotinamide...

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Autors principals: Trayer, Ian P., Trayer, Hylary R.
Format: Artigo
Idioma:Inglês
Publicat: 1974
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1168184/
https://ncbi.nlm.nih.gov/pubmed/4377106
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