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Discovery of a novel restriction endonuclease by genome comparison and application of a wheat-germ-based cell-free translation assay: PabI (5′-GTA/C) from the hyperthermophilic archaeon Pyrococcus abyssi

To search for restriction endonucleases, we used a novel plant-based cell-free translation procedure that bypasses the toxicity of these enzymes. To identify candidate genes, the related genomes of the hyperthermophilic archaea Pyrococcus abyssi and Pyrococcus horikoshii were compared. In line with...

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מידע ביבליוגרפי
Main Authors: Ishikawa, Ken, Watanabe, Miki, Kuroita, Toshihiro, Uchiyama, Ikuo, Bujnicki, Janusz M., Kawakami, Bunsei, Tanokura, Masaru, Kobayashi, Ichizo
פורמט: Artigo
שפה:Inglês
יצא לאור: Oxford University Press 2005
נושאים:
גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC1178009/
https://ncbi.nlm.nih.gov/pubmed/16040595
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gni113
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