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Investigation of the active site of papain with fluorescent probes
7-Chloro-4-nitrobenzo-2-oxa-1,3-diazole (NBD chloride) and 7-(2′-hydroxyethylthio)-NBD (obtained from NBD chloride and mercaptoethanol) undergo a reversible spectral change in alkaline solution that depends respectively on a single apparent pK(a) 9.76 (at 25°C) and 8.81 (at 32°C). In acid solution h...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
1973
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1177757/ https://ncbi.nlm.nih.gov/pubmed/4748829 |
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