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Purification and properties of dihydrofolate reductase from cultured mammalian cells

Dihydrofolate reductase was purified quickly and simply from small quantities of cultured mammalian cells by affinity chromatography. On gel electrophoresis of the purified enzyme, multiple bands of activity resulted from enzyme–buffer interaction at low but not high buffer concentration. A Ferguson...

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Détails bibliographiques
Auteurs principaux: Gauldie, Jack, Marshall, Lyse, Hillcoat, Brian L.
Format: Artigo
Langue:Inglês
Publié: 1973
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC1177704/
https://ncbi.nlm.nih.gov/pubmed/4723779
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