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Purification and properties of dihydrofolate reductase from cultured mammalian cells

Dihydrofolate reductase was purified quickly and simply from small quantities of cultured mammalian cells by affinity chromatography. On gel electrophoresis of the purified enzyme, multiple bands of activity resulted from enzyme–buffer interaction at low but not high buffer concentration. A Ferguson...

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Sonraí Bibleagrafaíochta
Main Authors: Gauldie, Jack, Marshall, Lyse, Hillcoat, Brian L.
Formáid: Artigo
Teanga:Inglês
Foilsithe: 1973
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC1177704/
https://ncbi.nlm.nih.gov/pubmed/4723779
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