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Properties of a 5′-nucleotidase purified from mouse liver plasma membranes

1. Extraction of a mouse liver plasma-membrane fraction with a detergent buffer, N-dodecylsarcosinate–Tris buffer (sarcosyl–Tris buffer), solubilized 90% of the protein and 70% of the 5′-nucleotidase activity. 2. The proteins of the sarcosyl–Tris buffer extract were fractionated by a rate-zonal cent...

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Bibliografiske detaljer
Main Authors: Evans, W. H., Gurd, James W.
Format: Artigo
Sprog:Inglês
Udgivet: 1973
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1177683/
https://ncbi.nlm.nih.gov/pubmed/4721620
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