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Preparation and properties of nexuses and lipid-enriched vesicles from mouse liver plasma membranes

Extraction of mouse liver plasma membranes with 4% (w/v) N-laurylsarcosinate–tris buffer, pH7.8, solubilized 80–90% of the protein and 60% of the 5′-nucleotidase activity. The membrane residue remaining after extraction was resolved on sucrose gradients into two fractions: a vesicular membrane fract...

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Detalhes bibliográficos
Main Authors: Evans, W. H., Gurd, J. W.
Formato: Artigo
Idioma:Inglês
Publicado em: 1972
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1173820/
https://ncbi.nlm.nih.gov/pubmed/4634836
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