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The reaction of a histidine residue in glutamate dehydrogenase with diethyl pyrocarbonate

1. One mol of diethyl pyrocarbonate will react with one mol of glutamate dehydrogenase polypeptide chains to form one mol of N(1)-carbethoxyhistidine. Reaction is prevented by NADH. 2. The 1:1 complex has an increased specific activity (1.4–2.0-fold). 3. The reason for the activation is discussed. T...

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Autors principals: Wallis, Robert B., Holbrook, J. John
Format: Artigo
Idioma:Inglês
Publicat: 1973
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1177682/
https://ncbi.nlm.nih.gov/pubmed/4352838
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