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Surrogate Splicing for Functional Analysis of Sesquiterpene Synthase Genes
A method for the recovery of full-length cDNAs from predicted terpene synthase genes containing introns is described. The approach utilizes Agrobacterium-mediated transient expression coupled with a reverse transcription-polydeoxyribonucleotide chain reaction assay to facilitate expression cloning o...
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| Principais autores: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society of Plant Biologists
2005
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1176406/ https://ncbi.nlm.nih.gov/pubmed/15965019 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1104/pp.105.059386 |
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