Kinetics of the reversible reaction of papain with 5,5'-dithiobis-(2-nitrobenzoate) dianion: evidence for nucleophilic reactivity in the un-ionized thiol group of cysteine-25 and for general acid catalysis by histidine-159 of the reaction of the 5-mercapto-2-nitrobenzoate dianion with the papain-5-mercapto-2-nitrobenzoate mixed disulphide.

Ítems similars

• The reaction of papain with Ellman's reagent [5,5′-dithiobis-(2-nitrobenzoate) dianion]
  per: Brocklehurst, K., et al.
  Publicat: (1972)
• A marked gradation in active-centre properties in the cysteine proteinases revealed by neutral and anionic reactivity probes. Reactivity characteristics of the thiol groups of actinidin, ficin, papain and papaya peptidase A towards 4,4'-dipyridyl disulphide and 5,5'-dithiobis-(2-nitrobenzoate) dianion.
  per: Brocklehurst, K, et al.
  Publicat: (1983)
• Reactions of papain and of low-molecular-weight thiols with some aromatic disulphides. 2,2′-Dipyridyl disulphide as a convenient active-site titrant for papain even in the presence of other thiols
  per: Brocklehurst, Keith, et al.
  Publicat: (1973)
• Reactivities of the various protonic states in the reactions of papain and of L-cysteine with 2,2'- and with 4,4'- dipyridyl disulphide: evidence for nucleophilic reactivity in the un-ionized thiol group of the cysteine-25 residue of papain occasioned by its interaction with the histidine-159-asparagine-175 hydrogen-bonded system.
  per: Brocklehurst, K, et al.
  Publicat: (1972)
• On the reactivity of metallothioneins with 5,5'-dithiobis-(2-nitrobenzoic acid).
  per: Li, T Y, et al.
  Publicat: (1981)