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The reaction of papain with Ellman's reagent [5,5′-dithiobis-(2-nitrobenzoate) dianion]

1. The report by Robyt et al. (1971) that the 2-nitro-5-mercaptobenzoate dianion (Nbs(2−)) produced by reaction of papain with the 5,5′-dithiobis-(2-nitrobenzoate) dianion (Nbs(2)(2−); Ellman's reagent) cleaves the three disulphide bonds in papain is shown to be incorrect. 2. When partially act...

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Detalhes bibliográficos
Main Authors: Brocklehurst, K., Kierstan, M., Little, G.
Formato: Artigo
Idioma:Inglês
Publicado em: 1972
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1173901/
https://ncbi.nlm.nih.gov/pubmed/4638793
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