A universal method to produce in vitro transcripts with homogeneous 3′ ends
A method is described that allows a general drawback of in vitro transcription assays to be overcome: RNA polymerases tend to add extra nucleotides to the RNA 3′ end that are not encoded in the linearized DNA template. Furthermore, these polymerases show a considerable rate of premature termination...
Uloženo v:
| Vydáno v: | Nucleic Acids Res |
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| Hlavní autoři: | , , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Oxford University Press
2002
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC117298/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12060694/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnf055 |
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