A universal method to produce in vitro transcripts with homogeneous 3′ ends

A method is described that allows a general drawback of in vitro transcription assays to be overcome: RNA polymerases tend to add extra nucleotides to the RNA 3′ end that are not encoded in the linearized DNA template. Furthermore, these polymerases show a considerable rate of premature termination...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Schürer, Heike, Lang, Kathrin, Schuster, Jens, Mörl, Mario
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 2002
Assuntos:	NAR Methods Online
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC117298/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12060694/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnf055
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