GTP hydrolysis by arf-1 mediates sorting and concentration of Golgi resident enzymes into functional COP I vesicles.

Upon addition of GTPgammaS to in vitro budding reactions, COP I vesicles form but retain their coat, making them easy to isolate and analyze. We have developed an in vitro budding assay that reconstitutes the formation of COP I-derived vesicles under conditions where GTP hydrolysis can occur. Once f...

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Detaylı Bibliyografya
Asıl Yazarlar: Lanoix, J, Ouwendijk, J, Lin, C C, Stark, A, Love, H D, Ostermann, J, Nilsson, T
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 1999
Konular:
Research Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC1171565/
https://ncbi.nlm.nih.gov/pubmed/10487746
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/emboj/18.18.4935
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