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Functional glycan-free adhesion domain of human cell surface receptor CD58: design, production and NMR studies.
A general strategy is presented here for producing glycan-free forms of glycoproteins without loss of function by employing apolar-to-polar mutations of surface residues in functionally irrelevant epitopes. The success of this structure-based approach was demonstrated through the expression in Esche...
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| Hauptverfasser: | , , , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
1999
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1171376/ https://ncbi.nlm.nih.gov/pubmed/10357807 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/emboj/18.11.2941 |
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