Crosslinking the EcoRV restriction endonuclease across the DNA-binding site reveals transient intermediates and conformational changes of the enzyme during DNA binding and catalytic turnover.

EcoRV completely encircles bound DNA with two loops, forming the entry and exit gate for the DNA substrate. These loops were crosslinked generating CL-EcoRV which binds and releases linear DNA only slowly, because threading linear DNA into and out of the DNA-binding 'tunnel' of CL-EcoRV is...

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Enregistré dans:
Détails bibliographiques
Auteurs principaux: Schulze, C, Jeltsch, A, Franke, I, Urbanke, C, Pingoud, A
Format: Artigo
Langue:Inglês
Publié: 1998
Sujets:
Research Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC1171021/
https://ncbi.nlm.nih.gov/pubmed/9822618
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/emboj/17.22.6757
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