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An examination of the utility of photogenerated reagents by using α-chymotrypsin

As a test of the labelling characteristics of photogenerated reagents, an aryl azide was photolysed in the aromatic-binding locus of a protein of known tertiary structure. The acyl-enzyme derived from the reaction of α-chymotrypsin with the p-nitrophenyl ester of p-azido[(14)C]cinnamate was isolated...

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Bibliografiske detaljer
Main Authors: Bridges, Alex J., Knowles, Jeremy R.
Format: Artigo
Sprog:Inglês
Udgivet: 1974
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1168435/
https://ncbi.nlm.nih.gov/pubmed/4462748
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