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An examination of the utility of photogenerated reagents by using α-chymotrypsin
As a test of the labelling characteristics of photogenerated reagents, an aryl azide was photolysed in the aromatic-binding locus of a protein of known tertiary structure. The acyl-enzyme derived from the reaction of α-chymotrypsin with the p-nitrophenyl ester of p-azido[(14)C]cinnamate was isolated...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1974
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1168435/ https://ncbi.nlm.nih.gov/pubmed/4462748 |
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