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Studies on the changes in protein fluorescence and enzymic activity of aspartate aminotransferase on binding of pyridoxal 5′-phosphate

1. The α and β subforms of aspartate aminotransferase were purified from pig heart. 2. The α subform contained 2mol of pyridoxal 5′-phosphate. The apo-(α subform) could be fully reactived by combination with 2mol of cofactor. 3. The protein fluorescence of the apo-(α subform) decreased non-linearly...

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Autors principals: Evans, Robert W., Holbrook, J. John
Format: Artigo
Idioma:Inglês
Publicat: 1974
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1168433/
https://ncbi.nlm.nih.gov/pubmed/4462747
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