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Molecular cloning and transcriptional analysis of the start gene CDC25 of Saccharomyces cerevisiae
To isolate the CDC25 gene of Saccharomyces cerevisiae we have transformed a cdc25-1, trp1 strain with a yeast gene bank constructed in YRp7 vector, selecting trp(+) clones able to grow at restrictive temperature. From several independent positive clones we have recovered a plasmid, called pDGEm-1, t...
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| Autors principals: | , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
1986
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1167121/ https://ncbi.nlm.nih.gov/pubmed/16453707 |
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