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Cryo-electron microscopy of vitrified chromosomes in situ.

Chromosomes of metaphase-arrested Chinese hamster ovary (CHO) and HeLa cells were examined in situ, unfixed and unstained, by cryo-electron microscopy. In hydrated, vitrified cryo-sections, chromosomes exhibit a characteristic homogeneous, grainy texture, which, on optical diffraction, gives rise to...

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Detalhes bibliográficos
Main Authors: McDowall, A W, Smith, J M, Dubochet, J
Formato: Artigo
Idioma:Inglês
Publicado em: 1986
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1166954/
https://ncbi.nlm.nih.gov/pubmed/3755397
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