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Evidence for light-induced 13-cis, 14-s-cis isomerization in bacteriorhodopsin obtained by FTIR difference spectroscopy using isotopically labelled retinals

We have obtained by Fourier transformed infra-red (FTIR)-spectroscopy BR-K, BR-L and BR-M difference spectra of bacteriorhodopsin regenerated with isotopically labelled retinals. Thereby, we are able to assign reliably the C(14)–C(15) and C=N stretching vibrations of the various intermediates. The l...

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Detalhes bibliográficos
Main Authors: Gerwert, Klaus, Siebert, Friedrich
Formato: Artigo
Idioma:Inglês
Publicado em: 1986
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1166862/
https://ncbi.nlm.nih.gov/pubmed/16453681
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