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A fluorimetric study of the role of calcium ions in the stability of thermolysin

1. Fluorimetric techniques were used to characterize the environment of tryptophan residues in thermolysin and apo-thermolysin. The apo-thermolysin was obtained by dissolving the enzyme in the presence of 10mm-EDTA, which removed the functional Zn(2+) ion and the four Ca(2+) ions/molecule from the e...

詳細記述

保存先:
書誌詳細
主要な著者: Fontana, Angelo, Vita, Claudio, Boccu', Enrico, Veronese, Francesco M.
フォーマット: Artigo
言語:Inglês
出版事項: 1977
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC1164937/
https://ncbi.nlm.nih.gov/pubmed/411488
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