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Modification of lactate oxidase with diethyl pyrocarbonate. Evidence for an active-site histidine residue

1. Diethyl pyrocarbonate inactivated l-lactate oxidase from Mycobacterium smegmatis. 2. Two histidine residues underwent ethoxycarbonylation when the enzyme was treated with sufficient reagent to abolish more than 90% of the enzyme activity, but analyses of the inactivation showed that the modificat...

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Bibliografiska uppgifter
Huvudupphovsmän: Soon, Choong Yee, Shepherd, Maxwell G., Sullivan, Patrick A.
Materialtyp: Artigo
Språk:Inglês
Publicerad: 1977
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Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC1164911/
https://ncbi.nlm.nih.gov/pubmed/921755
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