A convenient method of preparation of high-activity urease from Canavalia ensiformis by covalent chromatography and an investigation of its thiol groups with 2,2'-dipyridyl disulphide as a thiol titrant and reactivity probe.

Samankaltaisia teoksia

• Reactions of papain and of low-molecular-weight thiols with some aromatic disulphides. 2,2′-Dipyridyl disulphide as a convenient active-site titrant for papain even in the presence of other thiols
  Tekijä: Brocklehurst, Keith, et al.
  Julkaistu: (1973)
• UREASE DISTRIBUTION IN CANAVALIA ENSIFORMIS
  Tekijä: Granick, Sam
  Julkaistu: (1937)
• The mutability of stem bromelain: evidence for perturbation by structural transitions of the parameters that characterize the reaction of the essential thiol group of bromelain with 2,2'-dipyridyl disulphide.
  Tekijä: Brocklehurst, K, et al.
  Julkaistu: (1972)
• Purification, crystallization and preliminary X-ray analysis of urease from jack bean (Canavalia ensiformis)
  Tekijä: Balasubramanian, Anuradha, et al.
  Julkaistu: (2009)
• Preparation of fully active ficin from Ficus glabrata by covalent chromatography and characterization of its active centre by using 2,2'-depyridyl disulphide as a reactivity probe.
  Tekijä: Malthouse, J P, et al.
  Julkaistu: (1976)