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A sensitive method for measuring protein turnover based on the measurement of 2-3H-labelled amino acids in protein.

A method for measuring the rate of protein degradation is described. The method measures the change in 2-3H content of protein with time by racemization of the protein hydrolysate with acetic anhydride. The 3H on C-2 of amino acids is stable in proteins but becomes labile, owing to the action of tra...

詳細記述

保存先:
書誌詳細
主要な著者: Humphrey, T J, Davies, D D
フォーマット: Artigo
言語:Inglês
出版事項: 1976
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC1163789/
https://ncbi.nlm.nih.gov/pubmed/949338
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