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The rapid purification of 3-hydroxybutyrate dehydrogenase and malate dehydrogenase on triazine dye affinity matrices.

3-Hydroxybutyrate dehydrogenase (EC 1.1.1.30) and malate dehydrogenase (EC 1.1.1.37) were purified to homogeneity on a large scale involving only two sequential affinity-chromatography steps on two triazine dye-Sepharose matrices. Recoveries of both enzymes were in excess of 60%. Malate dehydrogenas...

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Main Authors: Scawen, M D, Darbyshire, J, Harvey, M J, Atkinson, T
Formáid: Artigo
Teanga:Inglês
Foilsithe: 1982
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC1158286/
https://ncbi.nlm.nih.gov/pubmed/6981412
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