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Solubilization and purification of rat liver 5'-nucleotidase by use of a zwitterionic detergent and a monoclonal-antibody immunoadsorbent.

1. A variety of detergents were used to solubilize 5'-nucleotidase from rat liver plasma membranes. 2. The zwitterionic detergent Sulphobetaine 14 gave optimal solubilization by the criteria of release into a high-speed-centrifugation supernatant and the formation of the smallest and least poly...

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Hlavní autoři: Bailyes, E M, Newby, A C, Siddle, K, Luzio, J P
Médium: Artigo
Jazyk:Inglês
Vydáno: 1982
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1158216/
https://ncbi.nlm.nih.gov/pubmed/6285894
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