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Expression of yeast deubiquitination enzyme UBP1 analogues in E. coli

BACKGROUND: It has been shown that proteins fused to ubiquitin undergo greater expression in E. coli and are easier to purify and renaturate than nonhybrid foreign proteins. However, there is no commercial source of large quantities of specific deubiquitinating proteases. This is the reason why hybr...

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Detalhes bibliográficos
Main Authors: Wojtowicz, Anna, Mazurkiewicz-Pisarek, Anna, Plucienniczak, Grazyna, Mikiewicz-Sygula, Diana, Chojnacka, Luiza, Lukasiewicz, Natalia, Plucienniczak, Andrzej
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2005
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1156937/
https://ncbi.nlm.nih.gov/pubmed/15924623
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2859-4-17
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