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A simple assay to study protein-mediated lipid exchange by fluorescence polarization.

We investigated the protein-mediated phospholipid transfer between small vesicles by fluorescence polarization measurements with diphenylhexatriene as optical probe. Thermotropic-phase-transition curves were taken after mixing two vesicle preparations of lipids exhibiting different gel-to-liquid pha...

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Detalhes bibliográficos
Main Authors: Xü, Y H, Gietzen, K, Galla, H J, Sackmann, E
Formato: Artigo
Idioma:Inglês
Publicado em: 1983
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1154080/
https://ncbi.nlm.nih.gov/pubmed/6847612
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