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Site-directed mutagenesis of beta-lactamase I. Single and double mutants of Glu-166 and Lys-73.

Two single mutants and the corresponding double mutant of beta-lactamase I from Bacillus cereus 569/H were constructed and their kinetics investigated. The mutants have Lys-73 replaced by arginine (K73R), or Glu-166 replaced by aspartic acid (E166D), or both (K73R + E166D). All four rate constants i...

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Sonraí Bibleagrafaíochta
Main Authors: Gibson, R M, Christensen, H, Waley, S G
Formáid: Artigo
Teanga:Inglês
Foilsithe: 1990
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC1149752/
https://ncbi.nlm.nih.gov/pubmed/1980064
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