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Isocitrate lyase from Phycomyces blakesleeanus. The role of Mg2+ ions, kinetics and evidence for two classes of modifiable thiol groups.

Isocitrate lyase was purified from Phycomyces blakesleeanus N.R.R.L. 1555(-). The native enzyme has an Mr of 240,000. The enzyme appeared to be a tetramer with apparently identical subunits of Mr 62,000. The enzyme requires Mg2+ for activity, and the data suggest that the Mg2(+)-isocitrate complex i...

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Bibliografiske detaljer
Main Authors: Rúa, J, de Arriaga, D, Busto, F, Soler, J
Format: Artigo
Sprog:Inglês
Udgivet: 1990
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1149708/
https://ncbi.nlm.nih.gov/pubmed/2268266
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