Pairing properties of bromouracil and repair of bromouracil-containing DNA. Possible utilization of bromodeoxyuridine triphosphate for site-directed mutagenesis.

5-Bromo-2'-deoxyuridine triphosphate (Br-dUTP) and dTTP are used interchangeably for DNA synthesis in vitro by the Klenow fragment of Escherichia coli DNA polymerase I. When DNA containing Br-dUMP instead of dTMP at a few preselected sites is transfected into competent bacteria, no mutation occ...

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Sonraí Bibleagrafaíochta
Main Authors: Muller, M, Martial, J, Verly, W G
Formáid: Artigo
Teanga:Inglês
Foilsithe: 1988
Ábhair:
Research Article
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC1149354/
https://ncbi.nlm.nih.gov/pubmed/3178734
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