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Effects of pH, detergent and salt on aggregation of Chinese-hamster-ovary-cell lysosomal enzymes.

Upon detergent or hypo-osmotic lysis of CHO-cell postnuclear supernatants or isolated lysosomes at pH 4.8, the lysosomal enzymes beta-hexosaminidase, beta-galactosidase, alpha-fucosidase and cathepsin C were readily pelleted, whereas the exogenous marker, long-term-internalized horseradish peroxidas...

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Detalhes bibliográficos
Main Authors: Buckmaster, M J, Ferris, A L, Storrie, B
Formato: Artigo
Idioma:Inglês
Publicado em: 1988
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1148797/
https://ncbi.nlm.nih.gov/pubmed/2965575
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