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Gene targeting using randomly inserted group II introns (targetrons) recovered from an Escherichia coli gene disruption library
The Lactococcus lactis Ll.LtrB group II intron retrohomes by reverse-splicing into one strand of a double-stranded DNA target site, while the intron-encoded protein cleaves the opposite strand and uses it to prime reverse transcription of the inserted intron RNA. The protein and intron RNA function...
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| Autori principali: | , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Oxford University Press
2005
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1148171/ https://ncbi.nlm.nih.gov/pubmed/15947133 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gki649 |
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