Purification and characterization of human hepatic cysteine-conjugate beta-lyase.

Cysteine-conjugate beta-lyase (EC 4.4.1.13) was purified about 880-fold from human liver obtained post mortem. The purification procedure included (NH4)2SO4 precipitation, chromatography on DEAE-cellulose and hydroxyapatite, gel filtration on Sephadex G-200, and chromatofocusing. The purified enzyme...

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Bibliographic Details
Main Authors: Tomisawa, H, Ichihara, S, Fukazawa, H, Ichimoto, N, Tateishi, M, Yamamoto, I
Format: Artigo
Language:Inglês
Published: 1986
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1146722/
https://ncbi.nlm.nih.gov/pubmed/3741406
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