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Purification and Characterization of West Nile Virus Nucleoside Triphosphatase (NTPase)/Helicase: Evidence for Dissociation of the NTPase and Helicase Activities of the Enzyme

The nucleoside triphosphatase (NTPase)/helicase associated with nonstructural protein 3 of West Nile (WN) virus was purified from cell culture medium harvested from virus-infected Vero cells. The purification procedure included sequential chromatography on Superdex-200 and Reactive Red 120 columns,...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Borowski, Peter, Niebuhr, Andreas, Mueller, Oliver, Bretner, Maria, Felczak, Krzysztof, Kulikowski, Tadeusz, Schmitz, Herbert
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2001
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC114115/
https://ncbi.nlm.nih.gov/pubmed/11238848
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.75.7.3220-3229.2001
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