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A two-step strategy for constructing specifically self-subtracted cDNA libraries

We have developed a new strategy for producing subtracted cDNA libraries that is optimized for connective and epithelial tissues, where a few exceptionally abundant (super-prevalent) RNA species account for a large fraction of the total mRNA mass. Our method consists of a two-step subtraction of the...

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Publicat a:Nucleic Acids Res
Autors principals: Laveder, Paolo, De Pittà, Cristiano, Toppo, Stefano, Valle, Giorgio, Lanfranchi, Gerolamo
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2002
Matèries:
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC113861/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11972353/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/30.9.e38
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