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Synthesis and recognition by DNA polymerases of a reactive nucleoside, 1-(2-deoxy-β-d-erythro-pentofuranosyl)-imidazole-4-hydrazide

We report the synthesis of a new nucleoside, 1-(2-deoxy-β-d-erythro-pentofuranosyl)-imidazole-4-hydrazide (dY(NH(2))) as a reactive monomer for DNA diversification. The 5′-triphosphate derivative (dY(NH(2))TP, 1) was evaluated in vitro as a substrate for several DNA polymerases. Primer extension rea...

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Main Authors: Strobel, Heike, Dugué, Laurence, Marlière, Philippe, Pochet, Sylvie
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 2002
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC113855/
https://ncbi.nlm.nih.gov/pubmed/11972322
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