Effect of acidotropic amines on the accumulation of newly synthesized membrane and luminal proteins in Chinese-hamster ovary (CHO) cell lysosomes.

Lysosomes were isolated by sequential gradient centrifugation [Madden, Wirt & Storrie (1987) Arch. Biochem. Biophys. 257, 27-38] from control or acidotropic-amine-treated Chinese-hamster ovary (CHO) cells. By marker-enzyme analysis, the preparation from chloroquine or NH4Cl-treated cells was abo...

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Bibliographic Details
Main Authors: Madden, E A, Storrie, B
Format: Artigo
Language:Inglês
Published: 1989
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Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1138441/
https://ncbi.nlm.nih.gov/pubmed/2730569
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