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Reconstitution of the folding pathway of collagen in a cell-free system: formation of correctly aligned and hydroxylated triple helices.

We describe here a cell-free system which will carry out the initial stages in the synthesis, post-translational modification and assembly of type-X collagen. The mRNA coding for bovine type-X collagen was synthesized in vitro and translated in a rabbit reticulocyte lysate to yield a protein that wa...

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Detalles Bibliográficos
Autores principales: Middleton, R B, Bulleid, N J
Formato: Artigo
Lenguaje:Inglês
Publicado: 1993
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC1137724/
https://ncbi.nlm.nih.gov/pubmed/8257444
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