Purification and characterization of multiple forms of the pineapple-stem-derived cysteine proteinases ananain and comosain.

A mixture of ananain (EC 3.4.22.31) and comosain purified from crude pineapple stem extract was found to contain numerous closely related enzyme forms. Chromatographic separation of the major enzyme forms was achieved after treatment of the mixture with thiol-modifying reagents: reversible modificat...

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Autores principales: Napper, A D, Bennett, S P, Borowski, M, Holdridge, M B, Leonard, M J, Rogers, E E, Duan, Y, Laursen, R A, Reinhold, B, Shames, S L
Formato: Artigo
Lenguaje:Inglês
Publicado: 1994
Materias:
Research Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC1137048/
https://ncbi.nlm.nih.gov/pubmed/8053898
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